A rapid biuret assay for protein of whole fatty tissues

Protein purification from whole tissues . Fatemeh Mehrpooyan*

Assay of cerebrospinal fluid protein: a rate biuret method evaluated.

We evaluated a rate colorimetric method (Beckman) for measuring total protein in cerebrospinal fluid. The automated instrument we used was Beckman's ASTRA TM. A 100-microL sample of spinal fluid is introduced into the biuret reagent in the reaction cell and the increase in absorbance at 545 nm is monitored for 20.5 s. Solid-state circuits determine the rate of alkaline biuret-protein chelate fo...

Assessment of a rapid immunochromatographic assay for the detection of avian influenza viruses

Rapid spreading of the low pathogenic avian influenza virus (AIV) caused by the H9N2 subtype and the highly pathogenic AIV caused by H5N1 have caused serious economic losses in the poultry industries of Asia. Therefore, the early detection of AIVs is crucial for the control of the disease. In the present study, the applicability of a rapid immunochromatographic (RIC) assay, which specifically d...

The assay of urinary peptides using a biuret reagent.

IT HAS BEEN POINTED OUT PREVIOUSLY that the method for the assay of urinary peptides using the biuret reagent is a much simpler procedure than others in current use (1). However, a disturbing difficulty with this method has been the lack of reproducibility of the biuret reagent (1). This resulted in sporadic errors approximately 10 per cent and in a feeling of uncertainty regarding the method i...

A biuret method for determination of protein in normal urine.

A biuret method has been developed which provides quantitative measurements of protein in normal urine without interference from drugs or pigments. This method is intended for use in monitoring clinical trials of new drugs-to detect nephrotoxicity. Protein is precipitated from duplicate samples of urine by addition of cold ethanolic phosphotungstic acid. The protein precipitates are separated b...